Abstracto
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Five guaianolides and a germacrolide were isolated from the leaf rinse extract of Viguiera gardneri (Asteraceae), together with known compounds. All compounds were detected in glandular trichomes collected from the leaves and were analyzed by HPLC. Structure elucidation was based on the analysis of spectroscopic data. Low energy conformations were obtained by quantum mechanical calculations. Three closely related guaianolides which were isolated as the main compounds were studied for their anti-inflammatory activity using the transcription factor NF-κB as molecular target. NF-κB DNA binding was inhibited at sesquiterpene lactones concentrations of 10 or 50 μM.
Sesquiterpene lactones of the guaianolide and germacrolide type are described, including anti-inflammatory activity and calculation of their low energy conformations, e.g. compound 1.