Abstracto
-
Dendritic cell-specific intercellular adhesion molecule-grabbing non-integrin (DC-SIGN), a specific C-type lectin expressed on DC, binds and transmits different pathogens to susceptible cells. In the present study, we examined the role of DC-SIGN in the capture of human papillomavirus (HPV) pseudovirions and activation of DC. We demonstrate that HPV virus-like particles (VLP) bind to DC-SIGN expressed on transfected Raji cells and that antibodies against DC-SIGN block this interaction. DC take up VLP, which activate expression of costimulatory markers and cytokines/chemokines. Although our results indicate that DC-SIGN is not the major receptor for VLP in DC, this interaction contributes to the activation of DC surface antigens (HLA class I) and of various cytokines/chemokines, particularly TNF-α, IL-6, and RANTES. Induction of these markers in DC by VLP was significantly abrogated when binding to DC-SIGN was blocked by anti-DC-SIGN antibodies. These results suggest that DC-SIGN has a functional role in DC activation induced by HPV-16 L1-VLP, and thus highlight new aspects of DC interactions with HPV VLP.
- Erosional convergent margins, where material is removed from the base of the upper plate and subducted on the lower plate, are fundamental features of the Circum Pacific. The erosional Middle America Trench convergent Pacific margin, remarkable for its broad diversity of dynamic environments, is a natural laboratory for studying convergent margin processes and seismogenesis. These environments include a shallow and deep trench axis, shallow‐to‐steep‐dipping plate interfaces, abnormally hot‐to‐cold subducting plate temperatures, and a subducting plate with smooth morphology bordering basement ridges and seamounts. The subducting topography accelerates erosion and localizes seismicity.