Actividades hemorrágica y coagulante del veneno de Bothrops alternatus de Argentina
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Abstracto
Argentina Bothrops alternatus venom was studied to determine its haemorrhagic and clotting activities. Mice were intradermical inoculated with different dilution of venom in physiologic solution of phosphate (pH 7.2) in a total volume of 0.1 ml, in order to determine haemorrhagic activity. After 2 hours, mice were sacrified with ether and the haemorrhagic area was measured. The Minimum Haemorrhagic Dose (MHD) is the amount of venom which induces an haemorrhagic area of 10 mm. MHD for B. alternatus venom was 3.6 μg. Serial dilutions of venom were confronted with human plasma in order to determine the Minimum Coagulant Dose. It was 14.5 μg/ml in 60 sec. As other species of Bothrops in Latinoamerica, Bothrops alternatus venom from Argentina has strong haemorrhagic and coagulant activities.