Samples of B. guineensis were collected, peeled, freeze-dried, homogenized by blender and subjected to extraction with 0.1% HCl in methanol. Purification phase was performed by high performance liquid chromatography (HPLC), using a binary linear gradient as mobile phase consisting of H2O-CH3COOH (97.5:2.5 v/v) and CH3OH with analytical reverse phase column Nucleosil C-18 Sigma-Aldrich, 250 x 4.6 mm, 5 μm. Two new anthocyanin pigments of B. guineensis fruit were purified and isolated, the structural information was obtained by spectroscopic methods: Ultraviolet-Visible (UV-Visible), Fourier Transform Infrared (FT-IR), Proton Nuclear Magnetic Resonance (1HNMR), Carbon-13 Magnetic Resonance (13C-NMR), one dimensional Total Correlation Spectroscopy (1D-TOCSY), Heteronuclear Multiple Bond Correlation (HMBC) and two dimensional Nuclear Overhauser Effect Spectroscopy (2DNOESY). The new anthocyanin pigments were identified as cyanidin 3-O-(6"-O-(a-Drhamnopyranosyl))- b-D-glucopyranose and 3-O-(4"-O-(a-D-rhamnopyranosyl))-b-Dglucopyranose. On the basis of these results a more widespread cultivation of B. guineensis plants, a wild tropical species, can be recommended because this fruit is a valuable source of natural food colorants which can promote many positive health benefits. Keywords: Güiscoyol, B. guineensis, Anthocyanin, Cyanidin, Arecaceae
