Population: unrelated, healthy, volunteer donors from Costa Rica. N: 1204 general Hispanic-admixed individuals, 72 Afro-Caribbeans, 59 Bribri-Cabecar Amerindians. Extraction: Chelex. PCR: commercial Amplitype PM+ HLA-DQA1 Typing Kit according to the manufacturer's recommendations (Perkin-Elmer, Norwalk, CT). Primer sequences and PCR conditions as in [1]. D1S80 was ampli®ed by PCR as in [2] with the AmpliFLP D1S80 Ampli®cation Kit (Perkin-Elmer) Typing: reverse dot-blot hybridizations as described by the manufacturer for PM and HLA-DQA1 [1]. Allele designations for D1S80 locus were determined by comparison of the sample fragments with the standard allelic ladder. Quality control: GEP±ISFH. Data analysis: Alfa [3], and Arlequin [4]. Access to the data:[email protected]Other remarks: The joint power of discrimination (PD)for these markers is 0.9999 and the a priori paternity chanceof exclusion (CE) is 0.9884 in the general Costa Rican population. The combined forensic PD is 0.99999 in Afro-Costa Ricans, and 0.999 in Amerindian Costa Ricans(Tables 1±3).
