HTS-based antioxidant evaluation of native plants of Kansas Artículo académico uri icon

Abstracto

  • Over 600 samples prepared from native Kansas plant biodiversity, were evaluated by High Throughput Screening (HTS) methods for enzymatic and non-enzymatic antioxidant potentials. The majority of the 111 species in 35 plant families examined have little or no reported antioxidant assessment. The enzymatic Antioxidant Response Element (ARE) assay determined the most potent species as Physalis longifolia, P. angulata (Solanaceae) and Impatiens capensis (Balsaminaceae) with EC50 values of 0.48 µg/mL, 1.93 µg/mL, and 3.22 µg/mL, respectively. The non-enzymatic Total Antioxidant Capacity (TAC) assay demonstrated that Mirabilis glabra (Nyctaginaceae), Larrea tridentata (Zygophyllaceae), and Eriogonum helichrysoides (Polygonaceae) were the most active antioxidant species with Trolox equivalent AUC values of 22.14, 22.13, and 21.10, respectively. Furthermore, these results, in conjunction with our previous P.longifolia study, support an Nrf2/ARE pathway and cell apoptosis relationship. The results of these experiments will be presented.

fecha de publicación

  • 1992