A simple and rapid analytical method, appropriate for quality control of repellent solutions containing DEET, was development and validated by high performance liquid chromatography. It was based on an isocratic elution in a Nucleosil 7 C18 column using a mobile phase composition of methanol and water (57:43 v/v); flow rate of 1.0 mL/min and the analyte was monitored at 220 nm. The linearity ranged between 15-75 µg/mL, the relative standard deviation (RSD) was 0.77% for intra and 1.31 % for inter-day precision. Recoveries of 100.2 and 102.0%. were observed. The method demonstrated to be rugged when applied in an e.quivalent chromatographic system and the excipients showed no interference in DEET retention time, hence specific
